高正彥 Cheng-Yen Kao

Education:

2009/9 ~ 2014/7

Ph.D., Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan.

2005/9 ~ 2007/7

M.S., Institute of Molecular Medicine, National Cheng Kung University, Tainan, Taiwan.

2001/9 ~ 2005/7

B.S., Department of Biochemical Science and Technology, National Chia Yi University, Chiayi, Taiwan.

Professional Experience:

2017/10 ~ present

Postdoctoral fellow, Department of Medical Microbiology and Immunology, University of Wisconsin-Madison (Dr. John-Demian (JD) Sauer Lab).

2016/8 ~ 2017/10

Postdoctoral fellow, Department of Biotechnology and Laboratory Science in Medicine, School of Biomedical Science and Engineering, National Yang Ming University, Taipei, Taiwan (Prof. Jiunn-Jong Wu Lab).

2014/8 ~ 2016/7

Postdoctoral fellow, Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan (Prof. Jiunn-Jong Wu Lab).

Research Interest & Expertise:

We are interested in understanding bacterial pathogenesis, antibiotic resistance, and drug development.

1. The majority of our work has focused on the H. pylori, an organism that infects 50% of people worldwide and is correlated with gastric cancer development. H. pylori adhesins and flagella-based motility play critical roles in initiation of successful infection, and as such my studies have focused on regulation of these adhesin and flagella-related genes. Currently, our research focuses on identifying novel genes involved in the bacterial metabolism and intracellular survival in Listeria monocytogenes. We have identified the regulon of a novel transcriptional regulator, Lmo1576 and implicated its regulation of metabolism in cytosolic survival of L. monocytogenes.

2. We have also collaborated with several other groups and clinical departments to survey the epidemiology of antimicrobial resistance in E. coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella spp., and Acinetobacter baumannii.

3. In addition, we have identified a number of small molecule inhibitors of bacterial PASTA kinases, a highly conserved family of proteins essential for virulence and antibiotic resistance in a variety of important pathogens including L. monocytogenes, Staphylococci, Enterococci and Mycobacteria, through the collaboration with Dr. Sauer (UW-Madison). In addition to developing these small molecules as novel antibiotics we are interested in understanding the signaling cascades downstream of the PASTA kinases that regulate antibiotic resistance and virulence, particularly in L. monocytogenes and methicillin resistant Staphylococcus aureus.


Journal papers

2019:

1. Jhp0106, a novel flagella-associated protein involved in the glycosylation of Helicobacter pylori major flagellin FlaA.

Kao CY*, Yang KY*, Wang S, Sheu BS, Hu ST, Wu JJ. (*equal contribution).

Helicobacter. 2019. (In preparation).

(SCI, IF: 4.123, 5-Year IF: 3.685, Ranking: 28/125 Microbiology)

2. Identification of novel OmpC variants contribute to carbapenem and cefepime resistance in Escherichia coli.

Chen LH, Kao CY, Yan JJ, Wu JJ.

Antimicrob Agents Chemother. 2019. (In preparation).

(SCI, IF: 4.255, 5-Year IF: 4.504, Ranking: 33/261 Pharmacology & Pharmacy)

3. Defeat antibiotic-resistant bacteria: exploring alternative drug targets for post-antibiotic era.

Wang CH, Kao CY.

J Biomed Sci. 2019. (In preparation).

(SCI, IF: 3.426, 5-Year IF: 3.322, Ranking: 46/133 Medicine, Research & Experimental)

4. battlefield between host and bacteria in the cytosol.

Kao CY, Sauer JD.

PLoS Pathog. 2019. (In preparation).

(SCI, IF: 6.158, 5-Year IF: 6.957, Ranking: 14/125 Microbiology)

5. Mutation of TrfA, a novel transcriptional regulator of fermentation, rescues cytosolic survival of 1,4-dihydroxy-2-naphthoate (DHNA) deficient Listeria monocytogenes.

Chen GY*, Kao CY*, Li AY, Sauer JD. (*equal contribution).

Infect Immun. 2019. (In preparation).

(SCI, IF: 3.256, 5-Year IF: 3.603, Ranking: 30/88 Infectious disease)

6. Fermented soymilk and soy and cow milk mixture, supplemented with orange peel fiber or Tremella flava fermented powder as prebiotics for high EPS-producing Lactobacillus pentosus SLC 13.

Huang ML, Huang JY, Kao CY, Fang TJ.

J Sci Food Agric. 2019. (In press).

(SCI, IF: 2.379, 5-Year IF: 2.537, Ranking: 8/57 Agriculture, Multidisciplinary)

2018:

7. Characterization of CRISPR-Cas systems in Clinical Klebsiella pneumoniae Isolates Uncovers its Potential Association with Antibiotic Susceptibility.

Li HY*, Kao CY*, Lin WH, Zheng PX, Yan JJ, Wang MC, Teng CH, Wu JJ. (*equal contribution).

Front Microbiol. 2018 Jul. doi: 10.3389/fmicb.2018.01595.

(SCI, IF: 4.019, 5-Year IF: 4.557, Ranking: 31/125 Microbiology)

8. Comparative Genomics of Escherichia coli Sequence Type 219 Clones from the Same Patient: Evolution of the IncI1 blaCMY-Carrying Plasmid in Vivo.

Kao CY, Chen JW, Liu TL, Yan JJ, Wu JJ.

Front Microbiol. 2018 Jul. doi: 10.3389/fmicb.2018.01518.

(SCI, IF: 4.019, 5-Year IF: 4.557, Ranking: 31/125 Microbiology)

9. Complete genome sequence of Lactobacillus pentosus SLC13, isolated from mustard pickles, a potential probiotic strain with antimicrobial activity against foodborne pathogenic microorganisms.

Huang ML, Huang JY, Kao CY, Fang TJ.

Gut Pathog. 2018 Jan 18;10:1. doi: 10.1186/s13099-018-0228-y. eCollection 2018.

(SCI, IF: 2.809, 5-Year IF: 3.015, Ranking: 54/125 Microbiology)

2017:

10. Inactivation of ferric uptake regulator (Fur) attenuates Helicobacter pylori J99 motility by disturbing the flagellar motor switch and autoinducer-2 production.

Lee AY*, Kao CY*, Wang YK, Lin SY, Lai TY, Sheu BS, Lo CJ, Wu JJ. (*equal contribution).

Helicobacter. 2017 Aug;22(4). doi: 10.1111/hel.12388. Epub 2017 Apr 12.

(SCI, IF: 4.123, 5-Year IF: 3.685, Ranking: 28/125 Microbiology)

11. Characterization of high exopolysaccharide-producing Lactobacillus strains isolated from mustard pickles for potential probiotic applications.

Huang JY, Kao CY, Liu WS, Fang TJ.

Int Microbiol. 2017 Jun;20(2):75-84. doi: 10.2436/20.1501.01.287.

(SCI, IF: 0.796, 5-Year IF: 1.645, Ranking: 116/125, Microbiology)

12. Molecular Characterization of Antimicrobial Susceptibility of Salmonella Isolates: First Identification of a Plasmid Carrying qnrD or oqxAB in Taiwan.

Kao CY*, Chen CA*, Liu YF, Wu HM, Chiou CS, Yan JJ, Wu JJ. (*equal contribution)

J Microbiol Immunol Infect. 2017 Apr;50(2):214-223. doi: 10.1016/j.jmii.2015.03.004. Epub 2015 May 14.

(SCI, IF: 2.094, 5-Year IF: 1.994, Ranking: 58/88 Infectious Diseases)

13. Complete Genome Sequence of Carbapenem-Resistant Klebsiella pneumoniae Strain 1756, Isolated from a Pus Specimen.

Kao CY, Yan JJ, Lin YC, Zheng PX, Wu JJ.

Genome Announc. 2017 Mar 30;5(13). pii: e00066-17. doi: 10.1128/genomeA.00066-17.

14. The Helicobacter pylori J99 jhp0106 gene, under the Control of the CsrA/RpoN Regulatory System, Modulates Flagella Formation and Motility.

Kao CY, Chen JW, Wang S, Sheu BS, Wu JJ.

Front Microbiol. 2017 Mar 28;8:483. doi: 10.3389/fmicb.2017.00483. eCollection 2017.

(SCI, IF: 4.019, 5-Year IF: 4.557, Ranking: 31/125 Microbiology)

15. Orange Peel Fiber and Tremella flava Chen Fermented Powder Effectively Induce Exopolysaccharide Production by Lactobacillus plantarum SLC 13.

Huang JY, Huang ML, Kao CY, Fang TJ.

Int. J. Agric. Biol. 2017. 19(3):437-444. doi: 10.17957/IJAB/15.0298.

(SCI, IF: 0.869, 5-Year IF: 0.943, Ranking: 28/56 Agriculture)

2016:

16. Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella spp. isolates in Mongolia.

Kao CY, Udval U, Huang YT, Wu HM, Huang AH, Bolormaa E, Yan JJ, Urangoo Z, Batbaatar G, Khosbayar T, Wu JJ.

J Microbiol Immunol Infect. 2016 Oct;49(5):692-700. doi: 10.1016/j.jmii.2015.05.009. (SCI, IF: 2.094, 5-Year IF: 1.994, Ranking: 58/88 Infectious Diseases)

17. Plasmid-mediated quinolone resistance determinants in quinolone-resistant Escherichia coli isolated from patients with bacteremia in a university hospital in Taiwan, 2001-2015.

Kao CY, Wu HM, Lin WH, Tseng CC, Yan JJ, Wang MC, Teng CH, Wu JJ.

Sci Rep. 2016 Aug 30;6:32281. doi: 10.1038/srep32281.

(SCI, IF: 4.122, 5-Year IF: 4.609, Ranking: 12/64 Multidisciplinary Sciences)

18. Overproduction of active efflux pump and variations of OprD dominate in imipenem-resistant Pseudomonas aeruginosa isolated from patients with bloodstream infection in Taiwan.

Kao CY*, Chen SS*, Hung KH, Wu HM, Hsueh PR, Yan JJ, Wu JJ. (*equal contribution)

BMC Microbiol. 2016 Jun 13;16(1):107. doi: 10.1186/s12866-016-0719-2.

(SCI, IF: 2.829, 5-Year IF: 3.066, Ranking: 53/125 Microbiology)

19. An integrated microfluidic system for diagnosis of the resistance of Helicobacter pylori to quinolone-based antibiotics.

Chao CY, Wang CH, Che YJ, Kao CY, Wu JJ, Lee GB.

Biosens Bioelectron. 2016 Apr 15;78:281-9. doi: 10.1016/j.bios.2015.11.046.

(SCI, IF: 8.173, 5-Year IF: 7.291, Ranking: 2/28 Electrochemistry)

20. First characterization of fluoroquinolone resistance mechanisms in CTX-M-producing Escherichia coli from Mongolia.

Kao CY, Udval U, Wu HM, Bolormaa E, Yan JJ, Khosbayar T, Wu JJ.

Infect Genet Evol. 2016 Mar;38:79-81. doi:10.1016/j.meegid.2015.12.010.

(SCI, IF: 2.545, 5-Year IF: 2.737, Ranking: 46/88 Infectious Diseases)

21. Helicobacter pylori Infection: An Overview of Bacterial Virulence Factors and Pathogenesis.

Kao CY, Sheu BS, Wu JJ.

Biomed J. 2016 Feb;39(1):14-23. doi: 10.1016/j.bj.2015.06.002. Epub 2016 Apr 1.

(SCI, IF: 1.723, Ranking: 235/292 Biochemistry & Molecular biology)

2015:

22. Intracellular Osteopontin Induced by CagA-positive Helicobacter pylori Promotes Beta-catenin Accumulation and Interleukin-8 Secretion in Gastric Epithelial cells.

Chang WL, Yang HB, Cheng HC, Yeh YC, Kao CY, Wu JJ, Lu CC, Sheu BS.

Helicobacter. 2015 Dec;20(6):476-84. doi: 10.1111/hel.12225. Epub 2015 Mar 3.

(SCI, IF: 4.123, 5-Year IF: 3.685, Ranking: 28/125 Microbiology)

23. The Challenge of Fluoroquinolone Resistance: Epidemiology, Mechanisms, and Clinical impact.

Kao CY, Wu JJ.

J Biomed Lab Sci. 台灣生檢雜誌.2015 Sep;27(3):77-86.

24. Carbapenem Resistance: Epidemiology, Mechanisms and Screening Methods.

Kao CY, Wu JJ.

J Biomed Lab Sci. 台灣生檢雜誌. 2015 Mar;27(1):1-9.

25. Genome Sequence and Annotation of Helicobacter pylori Strain Hp238 Isolated from a Taiwanese Patient with MALT Lymphoma.

Kao CY, Chen JW, Huang YT, Sheu SM, Sheu BS, Wu JJ.

Genome Announc. 2015 Feb 19;3(1). pii: e00006-15. doi: 10.1128/genomeA.00006-15.

2014:

26. CsrA regulates Helicobacter pylori J99 motility and adhesion by controlling flagella formation.

Kao CY, Sheu BS, Wu JJ.

Helicobacter. 2014 Dec;19(6):443-54. doi: 10.1111/hel.12148.

(SCI, IF: 4.123, 5-Year IF: 3.685, Ranking: 28/125 Microbiology)

27. The Complex Interplay among Bacterial Motility and Virulence Factors in Different Escherichia coli Infections.

Kao CY, Lin WH, Tseng CC, Wu AB, Cheng SW, Wang MC, Wu JJ.

Eur J Clin Microbiol Infect Dis. 2014 Dec;33(12):2157-62. doi: 10.1007/s10096-014-2171-2.

(SCI, IF: 2.537, 5-Year IF: 2.543, Ranking: 47/88 Infectious Diseases)

28. Dissemination of OXA-Carbapenemase-Producing Acinetobacter baumannii ST195 and ST642 in a Mongolian Hospital.

Kao CY, Udval U, Oyunchimeg R, Wu HM, Huang AH, Bolormaa E, Munkhtuya E, Yan JJ, Batbaatar G, Khosbayar T, Wu JJ.

Infect Genet Evol. 2014 Dec;28:313-6. doi: 10.1016/j.meegid.2014.10.024.

(SCI, IF: 2.545, 5-Year IF: 2.737, Ranking: 46/88 Infectious Diseases)

29. Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells.

Sheu SM, Cheng H, Kao CY, Yang YJ, Wu JJ, Sheu BS.

J Biomed Sci. 2014 Oct 9;21(1):96. doi:10.1186/s12929-014-0096-9.

(SCI, IF: 3.426, 5-Year IF: 3.322, Ranking: 46/133 Medicine, Research & Experimental)

30. Clinical and microbiologic characteristics of Klebsiella pneumonia from community-acquired recurrent urinary tract infections.

Lin WH, Kao CY, Yang DC, Tseng CC. Wu AB, Teng CH, Wang MC, Wu JJ.

Eur J Clin Microbiol Infect Dis. 2014 Sep;33(9):1533-9. doi: 10.1007/s10096-014-2100-4.

(SCI, IF: 2.537, 5-Year IF: 2.543, Ranking: 47/88 Infectious Diseases)

31. First Report of the Prevalence of Extended-Spectrum β-Lactamase Producing Escherichia coli in Two Mongolian Hospitals.

Kao CY*, Udval U*, Wu HM, Bolormaa E, Yan JJ, Khosbayar T, Wu JJ. (*equal contribution)

Infect Genet Evol. 2014 Jul;25:66-8. doi: 10.1016/j.meegid.2014.04.012.

(SCI, IF: 2.545, 5-Year IF: 2.737, Ranking: 46/88 Infectious Diseases)

32. Heteroresistance of Helicobacter pylori from the same patient prior to antibiotic treatment.

Kao CY, Lee AY, Huang AH, Song PI, Yang YJ, Sheu SM, Chang WL, Sheu BS, Wu JJ.

Infect Genet Evol. 2014 Apr;23:196-202. doi: 10.1016/j.meegid.2014.02.009.

(SCI, IF: 2.545, 5-Year IF: 2.737, Ranking: 46/88 Infectious Diseases)

2012:

33. Higher motility enhances bacterial density and inflammatory response in dyspeptic patients infected with Helicobacter pylori.

Kao CY, Sheu BS, Sheu SM, Yang HB, Chang WL, Cheng HC, Wu JJ.

Helicobacter. 2012 Dec;17(6):411-6. doi: 10.1111/j.1523-5378.2012.00974.x.

(SCI, IF: 4.123, 5-Year IF: 3.685, Ranking: 28/125 Microbiology)

34. Gemifloxacin can partially overcome quinolone resistance of H. pylori with gyrA mutation in Taiwan.

Chang WL, Kao CY, Wu CT, Huang AH, Wu JJ, Yang HB, Cheng HC, Sheu BS.

Helicobacter. 2012 Jun;17(3):210-5. doi: 10.1111/j.1523-5378.2012.00935.x.

(SCI, IF: 4.123, 5-Year IF: 3.685, Ranking: 28/125 Microbiology)

35. Length of thymidine homopolymeric repeats modulates promoter activity of sabA in Helicobacter pylori.

Kao CY, Sheu SM, Sheu BS, Wu JJ.

Helicobacter. 2012 Jun;17(3):203-9. doi: 10.1111/j.1523-5378.2012.00936.x.

(SCI, IF: 4.123, 5-Year IF: 3.685, Ranking: 28/125 Microbiology)

36. H. pylori clinical isolates have diverse babAB genotype distributions over different topographic sites of stomach with correlation to clinical disease outcomes.

Sheu SM, Sheu BS, Chiang WC, Kao CY, Wu HM, Yang HB, Wu JJ.

BMC Microbiol. 2012 May 30;12:89. doi: 10.1186/1471-2180-12-89.

(SCI, IF: 2.829, 5-Year IF: 3.066, Ranking: 53/125 Microbiology)

 

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